This newly launched core facility is focused on assessing physiological and metabolic phenotypes for novel mouse models of human disease which have been developed by our Center and Institute investigators. Such analyses provides insight into "on target" and "off target" tissue phenotypes, and will add substantial value towards understanding the physiological implications of changes in regulators of stem and progenitor cell formation. To complement assays for biochemical markers of skeletal and soft tissue metabolism, Promethion metabolic cages were recently acquired that provide 87 distinct readings per second on mouse activity, oxygen consumption, CO2 production, food intake, weight and water consumption.
The Physiology CORE provides "real" time data on metabolic phenotypes that fully complement biochemical studies. This output is coupled with dual energy X-ray absorptiometry that measures in vivo body composition cross-sectionally or longitudinally. A Seahorse XF24 analyzer that measures oxygen consumption, CO2 production and proton generation for cells or isolated mitochondria in a 24-well plate format is also available. XF24 provides bioenergetic data utilizing four agents in a mitochondrial stress test paradigm.
- Seahorse XF24 extracellular flux analyzer
- Promethion metabolic monitoring cage system
- PIXImus densitomer
- Beckman Coulter DXC 600 Pro Chemistry Analyzer
- Mesoscale Discovery Sector Imager
- Luminex 200 System
- Dynex Laboratories MRX Revelation Plate Reader
- Idexx ProCyte Dx hematology analyzer
Please use the Service Request Form prior to the start of work
For scheduling and inquiries, contact Victoria DeMambro