Staff:

Jeong Yoon , PhD
Core Director
207-885-8196
yoonje@mmc.org

Nancy Chandler-Conrey, MS
Core Manager
207-885-8269
chandn@mmc.org

Overview:

The Cell Culture and Viral Vector Core was established at MMCRI in 2004.  The objective of the core is to provide high quality, consistent cell culture and viral vector services to support research performed at MMCRI.  The Core is dedicated to the development and production of viral vectors for angiogenesis, stem cell & regenerative medicine and clinical renal research.  The Core operates on a fee-for-service basis and fees are established which will allow cost efficiency to investigators.  Fees cover costs only.

Viral vectors are produced in a dedicated BL2 facility within the MMCRI building.  Vector systems currently being used include adenovirus, retrovirus and lentivirus.

Services Available:

The Core facility currently offers the following services:

• Stock reagents for cell culture and viral preparations
• Provides established cell lines
• Transfection and transduction of gene-specific viral DNA provided by principal investigators.
• Large scale production, purification and titration of adenovirus, retrovirus, lentivirus.
• Technical assistance in cell culture and viral preparations.

Service Requests:

A Service Request Form can be downloaded from the website.  The form must be filled out completely with as much detailed information as possible.  The completed form must include the signature and account number of the Principal Investigator of the lab requesting services.  Completed forms should be submitted to Nancy Chandler-Conrey.