Director:
Calvin P.H. Vary, PhD
Core Director
(207) 885-8148
varyc@mmc.org
Overview:
Protein, Nucleic Acid Analysis and Cell Imaging Core at MMCRI has
been established to offer high quality, reproducible sequences and
characterization of peptides and nucleic acids.
Automated DNA sequencing is offered on both shorter and longer instruments
depending on the sample. DNA and RNA extraction is offered by robotic
extraction and purification. High throughput (16-capillary) sequencing
is available for appropriate projects.
Protein sequencing and post-translational analysis is offered using
Mass Spectrometry by means of protein digestion and subsequent peptide
fingerprinting and compositional analysis. A Matrix Assisted Laser
Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometer
is offered as well as a Liquid Chromatography and Quadrapole Mass
Spectrometer (LC/MS) for such sequencing. Post-translational modification
and interactor identification can also be accomplished.
Services Available:
Oligonucleotide
and siRNA ordering through IDT
DNA Sequencing:
(Service Request Form)
- DNA sequencing (run only)
- DNA sequencing (core does cleanup and run)
DNA/RNA
Robot use: (Service Request
Form)
- DNA robot (tails)
- DNA robot (plasmid)
- RNA robot
MALDI-TOF:
(Service Request Form)
- Mass
determination
- Protein
identification (eg. Interactor identification)
HPLC
and QSTAR: (Service Request Form)
- Nano-liquid chromatography
- Protein identification
- Protein post translational modification
- Peptide digestion, fingerprinting and sequencing
Service Requests:
Service
forms must be filled out completely with as much detailed information
as possible. The completed form must include the signature and
account number of the Principle Investigator of the lab requesting
services. Completed forms should be submitted to Kristin Riley
(Vary Lab).
This Core is supported by the COBRE Program of the National Center
for Research Resources.
