Course name: Instrumentation Techniques in Cell/Molecular Biology
Course number: BMB690
3 credits
Course coordinators:
Larry Fritz (UNE) lfritz@une.edu
Lucy Liaw (MMCRI) liawl@mmc.org
Meeting time: 2-3hrs/week at MMCRI, scheduled Monday 3-6pm at MMCRI
Instructors: MMCRI faculty

Class Materials. Password Protected!

This graduate-level course is designed for students enrolled in the Professional Science Master’s (PSM) program and bioscience Ph.D. programs and will introduce a number of high-end instruments and techniques in modern cell and molecular biology. A team of MMCRI principal investigators will assist in the course and each will lecture/demonstrate on his/her area of expertise. Topics covered include DNA analysis, mass spectrometry, histopathology, confocal microscopy, flow cytometry and cell sorting, transgenic and expression systems, and small animal magnetic resonance imaging techniques. For each new technique students will learn about theory, applications, limitations and have hands-on demonstrations. A common theme running through the course will emphasize stem cell biology. 

Date Lecture
1/28/08 MMCRI research overview, course description and requirements - DNA/protein analysis lecture
2/4/08 DNA/protein demo
2/11/08 tissue processing/histology
2/18/08 histopathology
2/25/08 flow cytometry/cell sorting lecture
3/3/08 flow cytometry demo
3/10/08 spring break UNE, no classes
3/17/08 microscopy/confocal microscopy
3/24/08 confocal microscopy demo
3/31/08 expression systems and embryonic stem cell lecture, 1.5hrs each
4/7/08 transgenic techniques
4/14/08 transgenics, demo
4/21/08 MRI lecture and demo
May 1-7 final exams


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