Igor Prudovsky , Ph.D.

Center for Molecular Medicine
Maine Medical Center Research Institute
Scarborough, ME 04074
(207) 885-8146 (phone)
(207) 885-8179 (fax)
prudoi@mmc.org

Biosketch

Igor Prudovsky received his Master’s degree from the Biology Department of Moscow University in 1975.  He received his Ph.D. and Doctor of Sciences (equivalent of Principal Investigator) degrees and became Professor of Molecular Biology at the Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences in Moscow, Russia respectively in 1979, 1991 and 1998.  His group studied the mechanisms of proliferation blockage in terminally differentiated cells of hematopoietic origin. In 1993-1995, he worked as a Visiting Scientist in the laboratory of Dr. Thomas Maciag at the Holland Laboratory of the American Red Cross in Rockville, MD.  Since 1998, he has been a Research Scientist at the Maine Medical Center Research Institute in the laboratory of Tom Maciag. Since 1999, Dr. Prudovsky is also heading the Southern Maine Regional Confocal Facility.  In March 2004, Dr. Prudovsky became a Principal Investigator at this Institute.

Research Interests

Our laboratory studies the mechanisms of non-classical protein export, primarily the release of FGF1 and IL1a, two ubiquitous pro-inflammatory and pro-angiogenic molecules. The major goals of this project is to understand how multiprotein release complexes are formed, how the released proteins are transported to the cell membrane and how they exit to the extracellular compartment without using the classical mechanism of exocytosis. The understanding of these processes may facilitate the development of new clinical approaches to the regulation of angiogenesis, inflammation and tumor growth.

Additionally, our laboratory studies the interaction between FGF/FGFR and Jagged(Delta)/Notch signaling, two major signaling systems involved in practically all of the aspects of organism development and organ formation. In particular, the downregulation of Notch signaling through the expression of soluble forms of Notch ligands induces FGF1 expression and release and the acquisition of the angiogenic phenotype by cells. The aim of the laboratory is to understand molecular mechanisms underlying the cross-talk between FGF and Notch signaling.

Selected Publications

Wong M.K.K., Prudovsky, I., Vary, C., Booth, C., Liaw, L., Mousa, S., Small, D., and Maciag, T., A Non- Transmembrane Form of Jagged-1 Regulates the Formation of Matrix-Dependent Chord-like Structures. Biochem. Biophys. Res. Commun., 2000; 268:853-859.

Landricina, M., Prudovsky, I., Mouta Carreira, C., Tarantini, F., Maciag, T., Amlexanox Reversibly Inhibits Cell Migration and Proliferation and Induces the Src-Dependent Disassembly of Actin Stress Fibers In Vitro, J. Biol. Chem, 2000; 275:32753-32762.

Prudovsky, I., Landriscina, M., Soldi, R., Bellum, S., Small, D., Andreeva, V., Maciag, T., Fusions to members of fibroblast growth factor gene family to study nuclear translocation and nonclassic exocytosis. Methods in Enzymology 2000; 327:369-382

Tarantini, F., Micucci, I., Bellum, S., Landriscina, M., Garfinkel, S., Prudovsky, I., and T. Maciag. The Precursor But Not the Mature Form of IL1 Blocks the Release of FGF1 in Response to Heat Shock, J. Biol. Chem., Rapid Commun., 2001; 276:5147-5151.

Landriscina, M., Soldi, R., Bagalá, Micucci, I., Bellum, S., Tarantini, F., Prudovsky, I., and Maciag, T. S100A13 Participates in the Release of Fibroblast Growth Factor 1 in Response to Heat Shock in Vitro. J. Biol. Chem., 2001; 22544-22552.

Lindner, V., Booth, C., Prudovsky, I., Small, D., Maciag, T., Liaw, L., Members of the jagged/Notch Gene Families are expressed in Injured Arteries and Regulate Cell Phenotype via Alterations in Cellmatrix and Cell-cell Interactions. Am. J. Pathol., 2001; 159: 875-883.

Landriscina, M., Bagala, C., Mandinova, A., Soldi, R., Micucci, I., Bellum, S, Prudovsky, I., and Maciag, T. Copper Induces the Assembly of a Multiprotein Aggregate Implicated in the Release of FGF1 in Response to Stress. J. Biol. Chem., 2001; 276:25549-25557.

Small, D., Kovalenko, D., Kacer, D., Liaw, L., Landriscina, M., Di Serio, C., Prudovsky, I., Maciag, T., Soluble Jagged 1 Represses the Function of Its Transmembrane Form to Induce the Formation of the Src-dependent Chord-like Phenotype; J. Biol. Chem., 2001; 276:32022-32030.

Mouta-Carreira, C., Landriscina, M., Prudovsky I and Maciag, T. The Comparative Release of FGF1 by Hypoxia and Temperature Stress. Growth Factors, 2001; 18:277-285.

Mandinov, L., Mandinova, A., Kyurkchiev, S., Kyurkchiev, D., Kehayov, I., Kolev, V., Soldi, R., Bagalá, C., deMuinck, E.D., Lindner, V., Post, M.J., Simons, M., Bellum, S., Prudovsky, I. and Maciag, T. Copper Chelation Represses the Vascular Response to Injury. Proc. Natl. Acad. Sci., U.S.A. 2003; 100:6700-6705.

Small, D., Kovalenko, D., Soldi, R., Mandinova, A., Kolev, V., Trifonova, R., Bagalá, C., Kacer, D., Liaw, L., Prudovsky, I. and Maciag, T. Notch Activation Suppresses FGF-Dependent Cellular Transformation. J. Biol. Chem. 2003; 278:16405-16413.

Mandinova, A., Bellum, S., Bagalá, C., Soldi, R., Landriscina, M., Tarantini, F., Prudovsky, I. and Maciag, T., S100A13 mediates the Copper-Dependent Stress-Induced Release of IL1 from Both Human U937 and Murine NIH 3T3 Cells. J. Cell Sci. 2003; 116:2687-2696.

Mandinov, L., Mandinova, A., Soldi, R., Graziani, I., Bagalá, C., Prudovsky, I, Maciag, T. Interleukin 1: The Choreographer for the Restenotic Ballet. Throm. & Haem., 2003; 90:369-371.

Prudovsky, I., Mandinova, A., Soldi, R., Bagalá, C., Graziani, I., Landriscina, M., Tarantini, F., Duarte, M., Bellum, S., Doherty, H. and Maciag, T. The Non-Classical Export Routes: IL1 and FGF1 Point the Way. J. Cell Sci. 2003; 118:4871-81.

Bagala, C., Kolev, V., Mandinova, A., Soldi, R., Mouta, C., Graziania, I., Prudovsky, I. and Maciag. T. The Alternative Translation of Synaptotagmin 1 Mediates the Non-Classical Release of FGF1. BBRC, 2003; 310(4):1041-7.

Lab Photo

Left to right: Radianna Trifonova, Igor Prudovsky, Raffaella Soldi, Olga Sideleva, Irene Graziani, Vihren Kolev, Doreen Kacer, Maria Duarte



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